Table 2.

Behavioral effects of exogenous allopregnanolone administration in rodents.

Published in	Spe-cies	Sex	Dose(s)	Route	Time before test	Region(s)	Behavioral test(s)	Main findings
Khisti and Chopde (2000)	mice	♂	1 × 0.5–2 mg/kg 1 × 0.5–2 μg/mouse	i.p. m.i.	30 min 15 min	•Syst. •CV	•FST	•0.5; 1; 2: ↓immobility •1; 2: ↓immobility
Khisti et al. (2000)	mice	♂	1 × 0.5–2 mg/kg 1 × 0.5–2 μg/mouse	i.p. m.i.	30 min 15 min	•Syst. •CV	•FST	•0.5; 1; 2: ↓immobility •1; 2: ↓immobility
Molina-Hernández et al. (2005)	rats	♀	21 × 0.5–2 mg/kg/d 1 × 1–4 μg/rat	s.c. m.i.	30 min 15 min	•Syst. •NAcc	•FST	•1.5; 2: ↓immobility (↑climbing) •3; 4: ↓immobility (↑climbing)
Rodríguez-Landa et al. (2007)	mice	♀OVX	1 × 0.5–3 mg/kg	i.p.	1 h	•Syst.	•FST •OFT	•1; 2; 3: ↓immobility
Pibiri et al. (2006)	mice	♂+♀	1 × 1.25–5 mg/kg	i.p.	30 min	•Syst.	•RIT	•ALLO (2.5; 5): ↓aggression
Naert et al. (2007)	rats	♂	1 × 0.05 mg/kg	i.p.	15–300 min	•Syst.	•FST	•No changes in the FST
Nin et al. (2008)	rats	♂	3 × 1.25–5 μg/rat	m.i.	24, 5, 1 h	•HPC	•FST	•2.5: ↓immobility
Rodríguez-Landa et al. (2009)	rats	♂	1 × 2 μg/rat	m.i.	immedia-tely	•HPCLS •HPCSF •HPCD	•FST •OFT	•HPCLS and HPCD: ↓immobility
Shirayama et al. (2011)	rats	♂	1 × 0.1 or 1 μg/rat	m.i.	4 d	•CV •HPCCA3 •HPCDG •PFC •NAcc •BLA •CeA	•LHT •OFT	•1: ↓lat./failure to escape ○ICV, HPCCA3 and CeA •HPCDG, PFC and BLA: no effect •No changes in the OFT
Evans et al. (2012)	rats	♂	50 mg released over 50 days	s.c.		•Syst.	•FST •NSFT	•↓immobility
Nin et al. (2012)	rats	♂	3 × 1.25–5 μg/rat	m.i.	24, 5, 1 h	•NAcc	•FST •M-Groom	•5: ↓immobility (↑climbing) •1.25; 5: ↓% correct transitions
Almeida et al. (2018)	rats	♂	3 × 5 μg/rat	m.i.	24, 5, 1 h	•CV	•FST	•↓immobility (↑swimming)
Almeida et al. (2019)	rats	♂	3 × 1.25–5 μg/rat	m.i.	24, 5, 1 h	•PFC	•FST •M-Groom	•No changes in the FST and M-Groom

Abbreviations and legends: increases (↑); decreases (↓); does not change (≌); ovariectomy (OVX); systemic (syst.); intraperitoneally (i.p.); subcutaneously (s.c.); microinjection (m.i.); hippocampus (HPC); dentate gyrus (DG); dorsal (D); lateral septum (LS); septofimbrial (SF); prefrontal cortex (PFC); central amygdala (CeA); basolateral amygdala (BLA); cerebral ventricle (CV); nucleus accumbens (NAcc); forced swim test (FST); open field test (OFT); resident-intruder test (RIT); learned helplessness test (LHT); novelty-suppressed feeding test (NSFT); microstructured grooming (M-Groom); latency (lat.).