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. 2020 Mar 18;39(10):e103111. doi: 10.15252/embj.2019103111

Figure EV3. CHK2‐mediated Beclin 1 phosphorylation promotes autophagy.

Figure EV3

  • A
    Immunoprecipitation of Bcl‐2 with Flag‐Beclin 1 WT, AA mutant, and DD mutant in HCT116 cells in normal medium or after HBSS starvation.
  • B
    Beclin 1‐depleted H1299 cells with reconstituted expression of the indicated proteins.
  • C, D
    Autophagy level was determined in Beclin 1‐depleted H1299 cells with reconstituted expression of Beclin 1 WT, AA mutant, or DD mutant in normal medium or after HBSS starvation in the presence (C) or absence (D) of CHK2.
  • E
    FIP200‐ and CHK2‐depleted H1299 cells with reconstituted expression of the indicated proteins.

Source data are available online for this figure.