Figure EV6. Generation and characterization of Pdi ^S359A/S359A mice.

• A
  Schematic diagram illustrating the single‐guide RNAs (sgRNAs) targeting intron 4 and intron 10 of mouse Pdi gene locus.
• B
  Detection of IRE1α, eIF2α, and PDI phosphorylation by immunoblotting in primary hepatocytes isolated from WT and Pdi ^S359A/S359A knock‐in (KI) mice treated with 5 μM Tg for 30 min.
• C, D
  Detection of serum creatinine (Cr) (C) and blood urea nitrogen (BUN) (D) levels of the same animals as in Fig 7C after 24 h of Tm injection. Data were shown as mean ± SEM from five biological replicates.
• E, F
  Hematoxylin and eosin staining of liver tissue of the same animals as in Fig 7C after 24 h of Tm injection (magnification × 200). Liver congestion was indicated with arrowheads (F). Scale bar = 100 μm. Representative images were shown from five animals per group analyzed.

Source data are available online for this figure.