Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
Cryo‐conserved lung tissues (human)	Lung Biobank Heidelberg
Air Liquid interface cultures (human)	Lung Biobank Heidelberg
Recombinant DNA
Antibodies
Uteroglobin (CC10)	BioVendor	RD181022220‐01
ß‐TUBULIN IV	Sigma‐Aldrich	T7941
Mucin 5AC	abcam	ab3649
KRT5	Sigma‐Aldrich	HPA059479
Oligonucleotides and other sequence‐based reagents
Chemicals, Enzymes and other reagents
DTT	Sigma‐Aldrich	D0632
Citric Acid Monohydrate	Sigma‐Aldrich	33114
Sucrose	Sigma‐Aldrich	S7903
RNase Inhibitor (40 U/µl)	Takara	634888
SUPERaseIn™, RNase Inhibitor (20 U/µl)	Thermo Fischer Scientific	AM2694
MgCl2 1M	Thermo Fischer Scientific	AM9539G
KCl 2M	Thermo Fischer Scientific	AM9640G
Nuclease free water	Thermo Fischer Scientific	AM9939
Hoechst 33258	Thermo Fischer Scientific	H3569
Tris buffer solution pH7.5 (1M) Ultrapure Grade	Thermo Fischer Scientific	15567027
DMEM/F12	Thermo Fischer Scientific	11320033
Sodium selenite	Sigma‐Aldrich	S5261
Ethanolamine	Sigma‐Aldrich	E0135
O‐Phosphorylethanolamine	Sigma‐Aldrich	P0503
Sodium Pyruvate (100 mM)	Thermo Fischer Scientific	11360039
Sodium Pyruvate	Gibco	11360070
Adenine	Sigma	A2786
HEPES	Thermo Fischer Scientific	15630106
GlutaMAX™ supplement	Thermo Fischer Scientific	35050038
Rock‐inhibitor/ Y‐27632	Stemcell Technologies	72302
0.25% Trypsin‐EDTA	ThermoFisher Scientific	25200056
Dispase	Corning	354235
DPBS	Thermo Fisher Scientific	14190094
Trypsin EDTA	Thermo Fisher Scientific	25200056
Dispase	Corning	354235
BSA	Sigma Aldrich	05479
PneumaCult‐ALI media	StemCell	05002
PneumaCult‐ALI 10x Supplement	StemCell	05003
Growth Medium SupplementPack	PromoCell	C‐39160
Software
Photoshop CS6	Adobe
Leica Application Suite	Leica
CellRanger 2.1.1	10× Genomics (https://github.com/10XGenomics/cellranger/releases/tag/2.1.0)
Seurat 3.0.0	https://www.nature.com/articles/nbt.4096 (https://github.com/satijalab/seurat)
Monocle 3	https://www.nature.com/articles/nmeth.4402 (https://github.com/cole-trapnell-lab/monocle3)
Python 3.7.1	Python Software Foundation (https://www.python.org/downloads/release/python-371/)
Statsmodels 0.9.0	https://www.statsmodels.org/stable/install.html
Scipy 1.4.1	https://www.scipy.org/scipylib/download.html
Other
Chromium™ SingleCell 3’ Library & Gel Bead Kit v2, 16 rxns	10× Genomics	PN‐120237
Chromium™ SingleCell 3’ Library & Gel Bead Kit v2, 4 rxns	10× Genomics	PN‐120267
Chromium™ SingleCell A Chip Kit, 48 rxns	10× Genomics	PN‐120236
Chromium™ SingleCell A Chip Kit, 16 rxns	10× Genomics	PN‐1000009
Chromium™ i7 Multiplex Kit, 96 rxns	10× Genomics	PN‐120262
Dounce homogenizer	Sigma	D8938‐1SET
Falcon 5 ml Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap (35 µm)	Corning	352235
Countess™ Cell Counting Chamber Slides	Thermo Fischer Scientific	C10314
Countess™ II Automated Cell Counter	Thermo Fischer Scientific	AMQAX1000
ThinCert™ Cell Culture Inserts 0.4 µm 12‐well plates	Greiner Bio‐One	665641
20 μm Cell Strainer	pluriSelect	43‐50020‐03
35 μm filter	Corning	352235