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. 2020 Apr 27;39(10):e102922. doi: 10.15252/embj.2019102922

Figure 2. Identification of potential GlpG and Rhom7 substrates.

Figure 2

  1. Workflow of the bioinformatic identification of candidate rhomboid substrates.
  2. A library of artificial substrates harbouring TMDs from 44 candidate rhomboid substrates.
  3. Western blot analysis of candidate substrates reproducibly cleaved by GlpG from the screen.
  4. Western blot analysis of candidate substrates reproducibly cleaved by Rhom7 from the screen.
  5. Venn diagram summarising putative rhomboid substrates identified by the screen.
Data information: In (C and D), rhomboid substrates that are uncleaved, cleaved by GlpG or cleaved by Rhom7 are marked by black, red and blue arrows, respectively. Controls, inactive enzymes and wild‐type Shigella sonnei (Ss). RecA, loading control. Source data are available online for this figure.