Figure EV2. GlpG and Rhom7 do not influence the growth of S. sonnei under various conditions.

• A, B
  Wild‐type S. sonnei or S. sonnei ΔglpGΔrhom7 were grown in liquid LB in the presence of oxygen at 37°C with shaking at 180 rpm. Growth was assessed by measuring the optical density of cultures at 600 nm (OD₆₀₀) (A) and colony‐forming units of bacteria (CFU) recovered from the same samples (B).
• C, D
  Bacteria were grown anaerobically in LB supplemented with 10 mM KNO₃ with shaking at 180 rpm. Growth was assessed by measuring the OD₆₀₀ as above (C) and CFU of bacteria recovered from the same cultures (D).
• E, F
  Comparisons of cellular respiration (NADH production) of S. sonnei ΔglpGΔrhom7 (green) to wild‐type S. sonnei (red) (E), or of S. sonnei ΔglpGΔrhom7 + glpG (green) to wild‐type S. sonnei (red) (F) in conditions provided by PM1‐10 of the Biolog MicroArrays. Areas of overlap are coloured yellow, while differences are shown as patches of red or green. Wild‐type S. sonnei seemed to respire better than S. sonnei ΔglpGΔrhom7 + glpG in pH 9.5 + anthranilic acid (black box in E). However, this result was not reproducible.

Data information: In (A‐D), data are presented as mean ± SD from three independent experiments.