Figure EV3. GlpG and Rhom7 do not affect S. sonnei survival following oxidative stress.

1. Resistance of wild‐type S. sonnei or S.sonnei ΔglpGΔrhom7 to H₂O₂. S. sonnei was grown in LB to an OD₆₀₀ of ≈ 0.5 and then pre‐incubated in 0 or 200 μM H₂O₂ for 20 min, before incubation in 0 or 2 mM H₂O₂ for 15 min. Bacterial survival was measured by plating samples to solid media. The percentage survival was calculated as the ratio of CFU recovered from H₂O₂‐exposed bacteria compared to bacteria not exposed to H₂O₂.
2. Resistance of wild‐type S. sonnei or S. sonnei ΔglpGΔrhom7 to paraquat (PQ). S. sonnei was grown as above and then pre‐incubated in 0 or 200 μM PQ for 15 min before incubation in 0 or 10 mM PQ for 45 min; samples were then plated to solid media to measure bacteria survival. The percentage survival was calculated as the ratio of CFU recovered from PQ‐treated bacteria to those that were untreated.

Data information: In (A) and (B), data are presented as mean ± SD from three independent experiments.