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T3SS‐mediated secretion of effectors by wild‐type S. sonnei and S. sonnei ΔglpGΔrhom7. Bacteria were grown to exponential phase (OD600 ≈ 0.5), and Congo red (final concentration, 200 μg/ml) was added to bacteria to induce secretion. Supernatants were analysed by SDS–PAGE and silver staining. Sizes of a molecular weight marker are shown in kDa.
