Table 1.
Strains used in this study. Unless stated otherwise, relevant genes are expressed from strong constitutive promoters.
| Name | Parental strain | Added DNA element | Relevant genotype | Source |
|---|---|---|---|---|
| CEN.PK113-5D | – | – | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 | Entian and Kötter (2007) |
| ST8251 | CEN.PK113-5D | pCfB2312 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 + pCfB2312 (Cas9) | Jessop-Fabre et al. (2016) |
| ST8940 | ST8251 | pCfB8794 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK | This study |
| ST8983 | ST8940 | BB3923 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH | This study |
| ST9016 | ST8983 | BB3939 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr | This study |
| ST9020 | ST8983 | BB3940 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: pTEF2→PcCpr | This study |
| ST9109 | ST8983 | BB3953 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-2:: CYB5-AtAtr2 | This study |
| ST9179 | ST9016 | PR-23852 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr ric1:: | This study |
| ST9316 | ST9179 | BB4020 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr ric1:: X-4:: ARO1-ARO2 | This study |
| ST9318 | ST9316 | pCfB9074 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr ric1:: X-4:: ARO1-ARO2 XII-4:: ARO4K229L-TRP2S65R,S76L | This study |
| ST9326 | CEN.PK113-5D | pCfB255 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 X-2:: URA3 | This study |
| ST9327 | ST8983 | pCfB255 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH X-2:: URA3 | This study |
| ST9328 | ST9016 | pCfB255 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr X-2:: URA3 | This study |
| ST9329 | ST9020 | pCfB255 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: pTEF2→PcCpr X-2:: URA3 | This study |
| ST9330 | ST9109 | pCfB255 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-2:: CYB5-AtAtr2 X-2:: URA3 | This study |
| ST9334 | ST9016 | pCfB9013 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr Ty-4::PcPsiK | This study |
| ST9335 | ST9016 | pCfB8796 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr Ty-4::PcPsiM | This study |
| ST9337 | ST8251 | pCfB8881 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XI-3:: CrTdc | This study |
| ST9346 | ST9337 | pCfB9073 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XI-3:: CrTdc XI-1:: PcCpr-PcPsiH | This study |
| ST9441 | ST9346 | pCfB8796 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XI-3:: CrTdc XI-1:: PcCpr-PcPsiH Ty-4:: PcPsiM | This study |
| ST9442 | ST9346 | pCfB9111 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XI-3:: CrTdc XI-1:: PcCpr-PcPsiH Ty-4:: BtAANAT | This study |
| ST9482 | ST9318 | pCfB8796 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: PcCpr ric1:: X-4:: ARO1-ARO2 XII-4:: ARO4K229L-TRP2S65R,S76LTy-4:: PcPsiM | This study |
| ST9649 | ST9327 | BB4335 | MATa Δura3 HIS3 LEU2 TRP1 MAL2-8c SUC2 XII-5:: PcPsiM-PcPsiK XI-3:: CrTdc-PcPsiH XI-1:: NCP1 X-2:: URA3 | This study |