Fig. 1.

Identification of cultured rat PBMSCs microscopy, immunocytochemical staining and flow cytometry techniques (A) Morphology of P3 PBMSCs; Osteogenesis, chondrogenesis, adipogenesis-committed differentiation of PBMSCs were determined by alizarin red, alcian blue and oil red staining, respectively (B) CD73, CD105, CD34 and HLA-DR of P3 PBMSCs treated with DAB and colored with hematoxylin, then observed under an inverted microscope (x100). Phenotypic analysis of PBMSCs analyzed by flow cytometry (C) CD29 FITC-A and CD90 FITC-A of P3 PBMSCs, together with the control isotypes Rat IgGM FITC-A and Rat IgG1 FITC-A detection (D) CD45 FITC-A, CD11b PE-A and CD79a PE-A of P3 PBMSCs, together with the control isotypes Rat IgG1 FITC-A, Rat IgG APE-A and Rat IgG1 PE-A detection. PBMSCs, peripheral blood-derived mesenchymal stem cells; P3, passage 3.