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. 2020 May 16;14:275–283. doi: 10.1016/j.reth.2020.03.008

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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Expression of relevant cytokines and genes associated with the balance of Th17/Treg under different culture conditions. The relevant cytokines detected by ELISA (A) Concentrations of TGF-β and IL-17 of the supernatant in lymphocytes and co-culturing group (mean ± SD, n = 6; ∗P < 0.05) (B) The Th17 associated (proinflammatory) genes, RORγt, IL-17 and IL-6 expressions of the lymphocyte as well as co-culturing groups were detected using reverse transcription-quantitative PCR assays (mean ± SD, n = 6; ∗P < 0.05) (C) The Treg associated (anti-inflammatory) genes, Foxp3, TGF-β and IL-10 expressions of the lymphocyte as well as co-culturing groups were detected using reverse transcription-quantitative PCR assays (mean ± SD, n = 6; ∗P < 0.05). TGF-β, transforming growth factor-β; IL, interleukin; RORγt, RAR-related orphan receptor γT; Foxp3, forkhead box p3.