Figure 2.

IGF2BP1 Is a Direct Target of Posttranscriptional Repression by miR-98-5p

(A) The predicted binding site for miR-98-5p of the IGF2BP1 3′ UTR. (B) Luciferase reporter assay was conducted in MSCs. Data are presented as averages from three independent experiments. (C and D) qRT-PCR (C) and western blot (D) were used to detect the mRNA and protein levels of IGF2BP1 (n = 6). (E) Cell apoptosis was analyzed by flow cytometry in siRNA-IGF2BP1-transfected MSCs (n = 6). All data are shown as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. ns, not significant.