Skip to main content
. 2020 May 1;20:764–776. doi: 10.1016/j.omtn.2020.04.013

Figure 4.

Figure 4

miR-98-5p Upregulates p53 through Inhibiting β-TrCP-Dependent Ubiquitination of p53

(A) qRT-PCR was used to detect the β-TrCP mRNA level in miR-98-5p-M transfected HC-MSCs and miR-98-5p-I transfected ITP-MSCs (n = 6). (B) β-TrCP and p53 were detected by western blot in miR-98-5p-M transfected HC-MSCs and miR-98-5p-I transfected ITP-MSCs (n = 6). (C) qRT-PCR was used to detect the β-TrCP mRNA level in siRNA-IGF2BP1 transfected MSCs (n = 6). (D) β-TrCP and p53 were detected by western blot in siRNA-IGF2BP1 transfected MSCs (n = 6). (E) β-TrCP-mediated p53 ubiquitination was detected in HC-MSCs and ITP-MSCs. Cells were treated with 20 μM proteasome inhibitor MG132 for 8 h, then p53 was immunoprecipitated and the ubiquitination of p53 was determined by western blot (n = 6). (F and G) β-TrCP-mediated p53 ubiquitination was detected in miR-98-5p-M-transfected HC-MSCs (n = 6) (F) and miR-98-5p-I-transfected ITP-MSCs (n = 6) (G). (H) The p53 ubiquitination was detected in siRNA-IGF2BP1-transfected HC-MSCs (n = 6). (I) The p53 ubiquitination was detected in miR-98-5p-I and siRNA-IGF2BP1 co-transfected ITP-MSCs (n = 6). All data are shown as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.