Fig. 1.

The kinetics of PEDV N protein synthesis and effects of Pan-caspase inhibitor on the N protein cleavage determined by Western Blot analysis (A) The kinetics of N protein. Confluent Vero cells were inoculated with PEDV 8aa (left lanes) or KD (right lanes) at an MOI of 0.1. Cell lysates were prepared at 24, 36 or 48 h post-inoculation (hpi). (B) Effects of Pan-caspase inhibitor on the N protein cleavage. Confluent Vero cells were inoculated with PEDV 8aa at an MOI of 0.1 in the presence of 100 u M Z-VAD-fmk(right lanes) or absence(left lanes). Cell lysates were collected at 24, 36, or 48 h post-inoculation (hpi). Uncleaved or cleaved N protein, designated as N or N′ and as β-actin were indicated by arrowheads.