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Cleavage of the PEDV N protein with recombinant caspase 3, 6 or 7. Expressed N protein was incubated with recombinant caspases with different dilution ratios (1:2 or 1:10) at 37 °C for 2 h. The mixtures were subjected to SDS-PAGE, and Western blot analysis was performed with the anti-HA monoclonal antibody. Uncleaved (N) and cleaved (N′) N protein are indicated by arrowheads.
