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. 2020 May 16;13(8):100790. doi: 10.1016/j.tranon.2020.100790

Figure 2.

Figure 2

NVP-AEW541–treated DCs decrease ovarian cancer cell migration. (A) THP-1 myeloid cells were treated with 5 μM of NVP-AEW541 for 1, 24, and 48 hours. Whole-cell lysates were resolved on SDS-PAGE and immunoblotted with tIGF1R and pIGF1R antibodies. Level of tubulin was used as a loading control. Cell migration was detected by wound scratch assay. (B, left panel) Human leukemic THP-1 cells were differentiated to DCs and treated with 5 μM of NVP-AEW541, after which they were co-cultured with SKOV3; scratch was applied 24 hours after cell merge. The growth of EOC cells into the scratch zone is demonstrated here at time 0, 24,and 48 hours after scratch; scale bar = 500 μm. (B, right panel) Scanning densitometry analysis of % migration in co-culture of SKOV3 with DCs. A value of 100% was given to % migration in untreated DCs at 0 hour. (C, left panel) THP-1 cells were differentiated to DCs and treated with 5 μM of NVP-AEW541, after which they were co-cultured with ES2; scale bar = 500 μm. (C, right panel) Scanning densitometry analysis of % migration in co-culture of ES2 with DCs. A value of 100% was given to the expression level of untreated DCs at 0 hour. The graphs represent average % migration of three independent experiments of ES2 and SKOV3 cells. Images were captured at a magnification of ×4. * P < .05. Bars represent SEM value.