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. 2019 May 8;12(4):305–317. doi: 10.1093/jmcb/mjz030

Figure 3.

Figure 3

Increased IL-1β induces miR-337-3p-mediated chondro-osteogenesis of rTDSCs under mechanical loading. (A) Enzyme-linked immunosorbent assay detected IL-1β in the supernatant medium of rTDSCs under mechanical loading (10%, 8 h/day) or not for 3 or 7 days. (B and C) Real-time PCR analysis of IL-1β (B), rno-miR-337-3p, Runx2, and Sox9 (C) in rTDSCs treated with 10 ng/ml IL-1β for 3 days. (D) Western blot analysis of Sox9 and Runx2 in rTDSCs treated with 10 ng/ml IL-1β for 3 days. The densitometric analysis of the proteins was normalized to GAPDH. Three independent experiments were analyzed for the bar graph below. (E) Alkaline phosphatase staining on Day 7 and Alcain blue staining on Day 14 of rTDSCs treated with IL-1β and rescued by rno-miR-337-3p mimics. Error bars, SEM (n = 3). *P < 0.05; **P < 0.01.