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. 2019 May 8;12(4):305–317. doi: 10.1093/jmcb/mjz030

Figure 5.

Figure 5

ERK1/2 pathway activated by IRS1 and JNK pathway activated by Nox4 induce chondro-osteogenic differentiation of TDSCs under mechanical loading. (A and B) rTDSCs were transfected with IRS1 silencing RNAs (A), Nox4 silencing RNAs (B), or negative control siRNA combined with mechanical loading or not for 3 days. Western blot analysis of indicated proteins was presented. (C) ERK pathway inhibitor U0126 was applied to rTDSCs under mechanical loading condition or not for 3 days. Protein level of Runx2 was tested. (D) JNK pathway inhibitor SP600125 was applied to rTDSCs under mechanical loading condition or not for 3 days. Protein level of Sox9 was tested. The densitometric analysis of the proteins was normalized to GAPDH. The lower bands of ERK, p-ERK, JNK, and p-JNK were used for densitometric analysis. Three independent experiments were analyzed for the bar graph below. *P < 0.05; **P < 0.01. Scale bar, 50 μm.