Figure 4.

Effects of siRNA treatment and IAV infection on cell viability. BCi-NS1.1 were incubated with siRNA for 3 days. For IAV PR8 infected cells, BCi-NS1.1 were incubated with IAV (MOI = 0.2) for 1 or 3 days. Staurosporine (10 µM) treated cells were used as a positive control for cytotoxicity. LDH activity of the supernatant was measured by using the LDH-Cytotoxicity Assay Kit. Cytotoxicity is expressed as the percentage of supernatant released LDH to LDH released from Staurosporine treated cells. Results are shown as the Mean ± SEM from three separate experiments.