Table 1.
Analytical performances of the strip test immunoassay and enzyme linked immunosorbent assay (ELISA), as resulted by validation experiments.
| Test Sample | Strip Test | ELISA | |
|---|---|---|---|
|
Blank (AFM1 ≤ 0.5 ng/kg) |
Mean response (ng/kg) | 4.2 | 8.2 |
| RSDr (%)1 | 93 | 16 | |
| RSDip (%)2 | 140 | 33 | |
| False suspect rate (%) | <0.1 | <0.1 | |
|
50% STC (25 ng/kg) |
Mean response (ng/kg) | 23.2 | 30.7 |
| RSDr (%) | 26 | 5 | |
| RSDip (%) | 32 | 5 | |
| False suspect rate (%) | 3 | <0.1 | |
|
STC (50 ng/kg) |
Mean response (ng/kg) | 53.1 | 57.9 |
| RSDr (%) | 12 | 5 | |
| RSDip (%) | 17 | 10 | |
|
150% STC (75 ng/kg) |
Mean response (ng/kg) | 85.6 | 105 |
| RSDr (%) | 16 | 15 | |
| RSDip (%) | 19 | 15 | |
| False negative rate (%) | 0.4 | 0.1 | |
| Cut-off value (ng/kg) | 37.7 | 47.5 |
1 RSDr relative standard deviation of the repeatability; 2 RSDip relative standard deviation for intermediate precision.