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. 2020 Mar 28;8(4):481. doi: 10.3390/microorganisms8040481

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Distribution of glycoside hydrolases (GHs) and their cellular localization in the selected Bifidobacterium species/strains. The circles represent the occurrence of enzymes (domains) classified in the respective GH families in the CAZy database (http://www.cazy.org). The extracellular and intracellular enzymes are shown by solid and open circles, respectively. Protein localization was predicted by signalP-5.0 (http://www.cbs.dtu.dk/services/SignalP/) and TMHMM Server v. 2.0 (http://www.cbs.dtu.dk/services/TMHMM/). The pseudogenes that encode incomplete protein sequences are excluded from the analysis. The GHs that are (possibly) involved in the degradation of HMOs and mucin O-glycans are colored in red.