Figure 3.

Small RNA responses to AEFV in female and male Ae. albopictus reproductive tissues and carcasses. The distribution of siRNA (21 nt) (A) and piRNA-like small RNAs (26-30 nt) (upper panel, B) mapped to the AEFV genome. A schematic illustration of the AEFV genome is shown on top of small RNAs mapping to the viral sequence. Yellow and blue bars represent positive- and negative-stranded reads, respectively. Regions with no coverage are indicated by gray bars. For AEFV-derived 26-30 nt piRNA-like small RNAs, relative nucleotide frequency at each position is shown as a heat map (lower panel in B) in which the color intensity denotes the frequency. The black and red arrowheads point to the 1U and 10A positions, respectively.