Figure 4.

Small RNA responses to SHTV in female and male Ae. albopictus reproductive tissues and carcasses. The distribution of siRNA (21 nt) (A) and piRNA-like small RNAs (26-30 nt) (upper panel, B) mapped to the SHTV genome. A schematic illustration of the SHTV genome is shown on top of small RNAs mapping to the viral sequence. Yellow and blue bars represent positive- and negative-stranded reads, respectively. Regions with no coverage are indicated by gray lines. For 26-30 nt SHTV-derived piRNA-like small RNAs, relative nucleotide frequency at each position is shown as a heat map (lower panel in B) in which the color intensity denotes the frequency. 26-30 nt small RNA reads were not detected in ovary samples (B). The black and red arrowheads point to the 1U and 10A positions, respectively.