Table 2.
Primers for the site substitutions in vip3Aa gene.
| Primers | Sequence 5′–3′ |
|---|---|
| P-3Aa-F | CATGCCATGGACATGAACAAGAATAATACTAAAT |
| P-3Aa-R | CGAGCTCTTACTTAATAGAGACATCGT |
| P-164P-R | TTCAGTAAGTGTaggGTTAATAAGTACA |
| P-164P-F | ATGTACTTATTAACcctACACTTACTG |
| P-164T-R | TTCAGTAAGTGTggtGTTAATAAGTACA |
| P-164T-F | GTACTTATTAACaccACACTTACTGAAA |
| P-164A-F | ACTTATTAACgcgACACTTACTG |
| P-164A-R | AGTAAGTGTcgcGTTAATAAGTA |
| P-152A-F | GATTTCTGATgcgTTGGATATTA |
| P-152A-R | ATAATATCCAAcgcATCAGAAAT |
| P-154A-F | TGATAAGTTGgcgATTATTAATG |
| P-154A-R | ATTAATAATcgcCAACTTATCAG |
Underlined sequences indicate the restricted enzyme sites of Nco I and Sac I. Lower case sequences indicate the mutant nucleotides in each primer.