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. 2020 Apr 20;12(4):266. doi: 10.3390/toxins12040266

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Modulatory activity of peptides Hcr 1b-3 and Hcr 1b-4 toward rASIC1a and rASIC3 channels. Acid-induced currents through rASIC1a (a,b) and rASIC3 (c,d) expressed in X. laevis oocytes were evoked by pH drop from 7.4 to 5.5 and 4.0; the effect of Hcr 1b-3 (a,c) and Hcr 1b-4 (b,d) at a concentration of 10 μM on the transient currents. Concentration-response curves for rASIC1a and rASIC3 indicating the inhibitory effect of peptides (e) and potentiating effect of Hcr 1b-4 on rASIC3 channels (f). Each point is means ± SD (obtained from 5 cells for each rASIC1a and rASIC3). Data were fitted by a logistic equation. The resulting values of the fitting parameters for rASIC1a: IC₅₀ 4.95 ± 0.19 μM; nH of 1.20 ± 0.05; A 30.3 ± 0.7% (Hcr 1b-3) and IC₅₀ 1.25 ± 0.04 μM; nH 1.08 ± 0.03; A 13.7 ± 0.8% (Hcr 1b-4). The resulting values of the fitting parameters for rASIC3: IC₅₀ 17 ± 5.8 μM; nH of 0.9 ± 0.2; A 26 ± 7%; (Hcr 1b-3) and EC₅₀ 1.53 ± 0.07 μM; nH 1.30 ± 0.06.; A 207.78 ± 1.56% (Hcr 1b-4).