Table 1.
Electrospray ionization positive (ESI)(+)-MS/MS parameters for detection of cardiac glycosides.
| Analyte | Precursor Ion (m/z) | Cone Voltage (V) | Product Ions (m/z) | Collision Energy (eV) |
|---|---|---|---|---|
| Oleandrin | 577.2 [M + H]+ | 30 |
373.21 433.1 |
15 10 |
| Digoxin | 781.2 [M + H]+ | 25 |
651.1 391.1 |
10 15 |
| Digitoxin | 782.4 [M + NH4]+ | 30 |
635.1 375.1 |
10 20 |
| Convallatoxin | 551.1 [M + H]+ | 20 |
369.2 351.2 |
10 20 |
| Ouabain | 585.1 [M + H]+ | 30 |
403.1 385.1 |
15 20 |
| Digoxin-d3 2 | 784.2 [M + H]+ | 25 |
654.1 394.2 |
10 15 |
1 Highlighted in bold: Most abundant product ion, 2 internal standard.