Table 3.

Validation data for cardiac glycosides in human urine.

Analyte	Reporting Limit (ng/mL)	Linear Range (ng/mL)	R2	Concentration Level	Recovery ± SD1	Repeatability (RSDr) (%)	Reproducibility (RSDwR) (%)	Measurement Uncertainty
				(ng/mL)	(%)			(%)
Oleandrin	0.1	0.1–20	0.9987	0.5	80 ± 13	3	18	36
				2.5	84 ± 9	3	13	26
				10	87 ± 5	2	7	13
					Mean: 84 ± 10	Mean: 3	Mean: 13
Digoxin	1	1–200	0.9993	5	93 ± 10	4	12	24
				25	92 ± 6	2	7	14
				100	92 ± 4	3	5	9
					Mean: 92 ± 7	Mean: 3	Mean: 8
Digitoxin	1	1–200	0.9975	5	86 ± 14	7	19	37
				25	95 ± 10	6	12	24
				100	88 ± 5	3	5	11
					Mean: 89 ± 11	Mean: 5	Mean: 12
Convallatoxin	1	1–200	0.9993	5	91 ± 15	4	19	36
				25	94 ± 7	2	8	26
				100	96 ± 3	1	3	16
					Mean: 94 ± 9	Mean: 3	Mean: 10
Ouabain	1	1–200	0.9994	5	87 ± 14	4	18	37
				25	89 ± 11	6	13	17
				100	93 ± 7	4	8	7
					Mean: 90 ± 11	Mean: 5	Mean: 13

¹ SD: Standard deviation.