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. 2020 Mar 31;12(4):385. doi: 10.3390/v12040385

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE of the Qm1 expression in fermentation. SDS-PAGE with Coomassie blue staining under reducing conditions after 6 h of induction. MWP: molecular weight pattern; SRF1, SRF2, and SRF3: rupture supernatants from each of the three fermentations of the Qm1 with SHuffle^® T7 Express clone; PRF1, PRF2, and PRF3: the pellet of rupture from each of the three fermentations of Qm1 SHuffle^® T7 Express clone; C-SR and C-PR: the rupture supernatants and pellet of rupture from the fermentation negative control sample. PRC +: the previous sample obtained positive detected by Western blot with an anti-his antibody. We used 20 µL per sample.