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. 2020 Mar 31;12(4):385. doi: 10.3390/v12040385

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Analysis of the antibody and antiviral response to PCV2 in pigs. (A) The immunization and viral challenge schedule. (B) IgG titer in serum measured in pigs vaccinated with Qm1 (grey) or the vehicle PBS (black) by indirect ELISA at the indicated days. (C) The pig serum was collected at day 60 and the viral titer was quantified in challenged pigs by qRT-PCR using SensiMixTM SYBR Hi-ROX kit (Bioline). The values are the mean ± standard error mean (SEM). n = 6, two-way ANOVA and the Sidak test for IgG titer and the Student’s t-test for viral titer ** p < 0.01; *** p < 0.001; **** p < 0.0001 vs. the control group.