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. 2020 Apr 9;12(4):425. doi: 10.3390/v12040425

Figure 1.

Figure 1

Reactive oxygen species (ROS) generation is attenuated in Huh7 cells expressing NS3/4A and NS5A. Total ROS production was determined in stably transfected Huh7 cells expressing the empty vector (A), Core (B), NS3/4A (C) and NS5A (D). Cells were plated in 6-well plates and 24 h post-seeding treated with menadione (50 μmol/L) for 6 h. In some experiments, cells were pre-treated with 5 mmol/L NAC 30 min prior to addition of menadione. ROS production was determined using flow cytometry and the relative main signal intensity is depicted. The graphs show means ± standard deviation (SD) of three independent experiments. A t-test was performed to compare the means and the asterisks represent the p value ** <0.0093 and * <0.0127. n.s = non-significant, (p values > 0.05 are considered not statistically significant).