Skip to main content
. 2020 Apr 9;12(4):425. doi: 10.3390/v12040425

Figure 5.

Figure 5

HCV Core and NS5A are preferentially degraded after menadione treatment. LC3-I/II and p62 autophagy markers were detected by Western blotting in Huh7 cells stably expressing the empty vector (A), Core (B) NS3/4A (C) and NS5A (D) HCV proteins. Non-treated cells (−/−) were used as control. Oxidative stress was induced by 50 μmol/L menadione (+/−) and in some experiments cells were treated with 5 mmol/L NAC (+/+). Protein band intensities were quantified using ImageLab software (BioRad). The relative protein expression was calculated based on the expression of GAPDH and compared to the expression of the control cells. The graphs depict means ± SD of three independent experiments. A t-test was performed to compare the means and the asterisks represent the p value *** <0.0007, ** ˂0.0072 and * ˂0.04. (p values > 0.05 are considered not statistically significant).