Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Apr 2;12(4):395. doi: 10.3390/v12040395

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Immunoblots of transfected cells and concentrated VSV pseudotypes. (A) Expression of arenavirus GPCs in HEK293FT. The proteins separated on SDS-PAGE and transferred onto nitrocellulose were probed with an anti-HA antibody. (B) Anti-HA immunoblot of biotinylated plasma membrane proteins from cells transfected with arenavirus GPCs. (C) Anti-HA immunoblot of HEK293FT cells transfected with arenavirus GPCs at 3 days post VSV inoculation. In A to C, the intact GPCs migrate at approximately 60–75 kDa and the cleaved GP2s between 10 and 37 kDa, as indicated. Anti-FLAG antibody served for ABV-1 GPC detection from the same membrane, but due to differences in the band intensity the anti-FLAG staining is shown as a strip overlaid on top of the anti-HA staining. M = molecular weight marker (Precision Plus Protein Dual Color Standards, Bio-Rad). Odyssey Infrared Imaging System (LI-COR) served to record the results.