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. 2020 Mar 30;12(4):378. doi: 10.3390/v12040378

Figure 4.

Figure 4

The stability and antiviral function of A3H haplotype II is dominant over the destabilizing N15del mutation of haplotype III. (A) Protein schematic of A3H chimeras used in this study. Haplotypes are denoted as I, II, or III in the N-terminal (N) or C-terminal (C) end of the flexible linker. Yellow boxes denote unstable haplotypes, and blue boxes represent active haplotypes. (B) Immunoblot of indicated wild-type (WT) A3H haplotypes or chimeras expressed in 293T cells. Western blotting of whole cell lysates was performed using anti-A3H and anti-Lamin B1 as a loading control. (C) Immunofluorescence imaging of HeLa cells transfected with A3H chimeras III–II, or II–III. Cells were stained for fluorescence microscopy using anti-A3H (green), and DAPI to detect the nucleus (blue). Images are representative of 30 randomly selected field images across three independent experiments.