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. 2020 Apr 10;12(4):429. doi: 10.3390/v12040429

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunoblotting detection of BoHV-1 (A), PRV (B), and HSV-1 gB (C) in cell lysates (cl) of constructed cell lines or SEC-isolated EVs. (+): gB-expressing cells; (−): MJSpuro, MDBKpuro, SK6puro, or untransduced Jurkat cells (Ø) were used as negative controls. Virus-infected cell lysates (cl) were analyzed as positive controls; in the case of BoHV-1, gB was immunoprecipitated (IP) with H2 antibody before immunoblotting with anti-BoHV-1 serum. Size markers are in kilodaltons. gBa: uncleaved BoHV-1 and PRV gB precursor; gBb: cleaved N-terminal gB subunit; gBc: cleaved C-terminal gB subunit.

Immunoblotting detection of BoHV-1 (A), PRV (B), and HSV-1 gB (C) in cell lysates (cl) of constructed cell lines or SEC-isolated EVs. (+): gB-expressing cells; (−): MJSpuro, MDBKpuro, SK6puro, or untransduced Jurkat cells (Ø) were used as negative controls. Virus-infected cell lysates (cl) were analyzed as positive controls; in the case of BoHV-1, gB was immunoprecipitated (IP) with H2 antibody before immunoblotting with anti-BoHV-1 serum. Size markers are in kilodaltons. gBa: uncleaved BoHV-1 and PRV gB precursor; gBb: cleaved N-terminal gB subunit; gBc: cleaved C-terminal gB subunit.