. 2020 Apr 15;8(4):568. doi: 10.3390/microorganisms8040568

Table 1.

Primers and conditions used for the amplification of virulence factors genes.

Target	Primer	Sequence (5′-3′)	Size (bp)	T (°C)	Reference
fimA	fimA-Fw	GTTGTTCTGTCGGCTCTGTC	447	55	[21]
	fimA-Rv	ATGGTGTTGGTTCCGTTATTC	447	55	[21]
papGIII	papG-Fw	CATTTATCGTCCTCAACTTAG	482	55	[21]
	papG-Rv	AAGAAGGGATTTTGTAGCGTC	482	55	[21]
papC	papC-Rw	GACGGCTGTACTGCAGGGTGTGGCG	382	63	[20]
	papC-Rv	ATATCCTTTCTGCAGGGATGCAATA	382	63	[20]
aer	aer-Fw	TACCGGATTGTCATATGCAGACCGT	602	63	[20]
	aer-Rv	AATATCTTCCTCCAGTCCGGAGAAG	602	63	[20]
hlyA	hlyA-Fw	AACAAGGATAAGCACTGTTCTGGCT	1117	63	[20]
	hlyA-Rv	ACCATATAAGCGGTCATTCCCGTCA	1117	63	[20]
cnfI	cnfI-Fw	AAGATGGAGTTTCCTATGCAGGAG	498	63	[20]
	cnfI-Rv	CATTCAGAGTCCTGCCCTCATTATT	498	63	[20]