Table 1.
Genes | Direction | Sequences (5′-3′) |
---|---|---|
Rv1964 | F-BamH I | GCCAAGACAATTGCGGATCCATGGTAATCGTGGCCGACAAG |
R-EcoR V | TGGTGGTGGTGGTGGATATCTCAGGACACCATGAATGGGATG | |
IL-6 | Sense | TGCCTTCTTGGGACTGAT |
Antisense | CTGGCTTTGTCTTTCTTGTT | |
TNF-α | Sense | CGATGAGGTCAATCTGCCCA |
Antisense | CCAGGTCACTGTCCCAGC | |
IL-1β | Sense | GCTAGGCTGCTGAGGTTTCTT |
Antisense | TGAAATGCCACCTTTTGACAG | |
β-actin | Sense | TGCTGTCCCTGTATGCCTCT |
Antisense | GGTCTTTACGGATGTCAACG |
Notice: The underscores were the sites for restriction digest.