Figure 2.

Characteristics of adipose‐derived mesenchymal stem cell (ASCs). ASCs were confirmed on the basis of colony formation, adipogenesis and osteogenesis. (a) They were stained with crystal violet to confirm colony formation. (b) The fourth passage ASCs were stained with Oil Red O to detect adipogenesis (scale bar, 50 μm). (c) They were stained with alizarin red S to detect osteogenesis (scale bar, 50 μm). (d–h) Cell surface markers of mesenchymal stem cells were evaluated using flow cytometry. The surfaces of ASCs were positive for CD29 and CD90 and negative for CD11b, CD31 and CD45