Comparison of the antigenicity and hydrophilicity of the HN of selected PPMV-1 and LaSota vaccine. Protean module of Laser gene was used to describe and analyze the antigenicity and hydrophilicity of hemagglutinin-neuraminidase (HN). GXG3 and GXG35 are representative isolates of sub-genotype VI.2.1.1.2.2, GXG2 is a representative isolate of sub-genotype VI.2.1.1.2.1, and LaSota vaccine is representative of genotype II vaccines. The pink diagram represents the antigenicity analysis of the HN, and the blue diagram represents the hydrophilicity analysis of the HN. The rectangular areas highlight the antigenicity and hydrophilicity differences in the HN between different viruses. (A) 1. HN helical folding structures: region 1 (175–228 aa); region 2 (237–288 aa); region 3 (316–396 aa); region 4 (401–443 aa); region 5 (472–515 aa); and region 6 (521–577 aa); (B) signal peptide region in cytoplasm (tail, 1–26 aa); 2. transmembrane region (rod-shaped, 27–48aa); 3. extracellular amino acids region (spherical head, 49–75aa).