Figure 4.

Separation of tryptic peptides from native and semi-carbazide-modified CTXs. Tryptic hydrolysates of reduced and s-carboxymethylated (RCM)-CTX1, RCM-SEM-CTX1, RCM-CTX3, and RCM-SEM-CTX3 were applied on a Discovery BIO wide pore C18 column (4.6 mm × 25 cm) equilibrated with 0.1% TFA was eluted with a linear gradient of 5–50% acetonitrile for 50 min. The flow rate was 0.8 mL/min, and the effluent was monitored at 230 nm.