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. 2020 Apr 18;12(4):262. doi: 10.3390/toxins12040262

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of CTX1, SEM-CTX1, CTX3, and SEM-CTX3 on the permeability of egg-yolk phosphatidylcholine/egg-yolk sphingomyelin (EYPC/EYSM) and EYPC/EYSM/cholesterol (Chol) vesicles. The signal was expressed as the percentage of total calcein release after addition of 0.2% Triton X-100. (A) CTX3 concentration-dependently induced the release of calcein from EYPC/EYSM/Chol vesicles. (Inset) Membrane-damaging activity of 500 nM CTX1, SEM-CTX1, CTX3, and SEM-CTX3 on EYPC/EYSM/Chol (* p < 0.05). (B) CTX1 and SEM-CTX1 concentration-dependently induced the release of calcein from EYPC/EYSM vesicles. (Inset) Membrane-damaging activity of 500 nM CTX1, SEM-CTX1, CTX3, and SEM-CTX3 on EYPC/EYSM vesicles (* p < 0.05).