Figure 3.

HIV-1 AD8 Nef antagonises tetherin in primary macrophages. (A) MDMs were infected with AD8 for seven days and cell surface tetherin levels quantified by flow cytometry. Uninfected cells were immunolabelled with anti-HRP as a staining control. The left-hand panel shows the result from a representative experiment; the right-hand panel shows the average relative tetherin levels from five donors ± SEM with relevant p-values; (B) MDMs were infected with HIV-1 AD8 for seven days. Virus and the corresponding cells were lysed and analysed by western blotting. The top panel shows representative western blots. The graphs in the bottom panels show the average relative cell (left) and virus (right) p24 levels from four donors ± SEM with relevant p-values.