Figure 5.

N- and C-terminal domains contribute to HIV-1 AD8 Nef’s anti-tetherin activity. HeLa cells were transfected with AD8 Nef, HIV-1 NL4.3 Nef or chimeric Nef proteins that contained the N-terminal 85 residues of AD8 Nef and the C-terminal 122 residues of NL4.3 Nef (AD8-NL4.3 Nef) or vice versa (NL4.3-AD8 Nef). Two days post-transfection, cell surface tetherin levels were quantified by flow cytometry. Mock-transfected cells were immunolabelled with anti-HRP as a staining control. The left-hand panel shows the results of a representative experiment; the right-hand panel shows the average relative tetherin levels from four independent experiments ± SEM with relevant p-values.