Figure 7.

The Nef proteins of different HIV strains show varying degrees of anti-tetherin activity. HeLa cells were transfected with IRES GFP plasmids encoding Nef proteins from a number of HIV strains, and cell surface tetherin levels were quantified by flow cytometry. Mock-transfected cells were immunolabelled with anti-HRP as a staining control. The upper panels and lower left-hand panel show the result from a representative experiment; the lower right-hand panel shows the average relative tetherin levels from four independent experiments ± SEM.