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. 2020 Mar 27;12(4):371. doi: 10.3390/v12040371

Figure 1.

Figure 1

Pol II Purification. (A) Elution from the DEAE Sepharose FF column contains nuclease activity as demonstrated by RNA degradation in lane DEAE. The elution from DEAE was further purified using the SP Sepharose FF column to remove the nuclease activity, as shown in the SP lane. l-PSTVd, 480 ng linear PSTVd; (B) Purified protein factions were visualized in an SDS-PAGE gel after coomassie blue staining. Protein bands corresponding to the putative Pol II subunits were labeled based on the molecular weight of the corresponding homologs in Arabidopsis. See Table S1 for details; (C) Immunoblots detection of the largest subunit of Pol II, Rbp1, using the 8WG16 antibody. Input, raw lysate of wheat germ. DEAE, the elution fraction from the DEAE Sepharose FF column. SP, the elution fractions (1 and 2, elution using 0.15 M and 0.3 M ammonium sulfate, respectively) from the SP Sepharose FF column. Buffer, protein storage buffer.