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. 2020 Mar 27;12(4):371. doi: 10.3390/v12040371

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Pol II-catalyzed transcription using PSTVd circular RNA templates. (A) In vitro transcription products detected by the (+)-PSTVd riboprobe; (B) The PSTVd templates detected by the (−)-PSTVd riboprobe. Lanes 1–4 were supplied with circular PSTVd as templates, while lane 5 was supplied with linear PSTVd as the template. c, circular PSTVd. l, linear PSTVd.