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PSTVd replication in protoplasts. Top panel shows the schematic presentation of T-DNA disruption in the TFIIS locus and the genotyping result. The genotyping PCR, using two sets of primers (P1+P3 and P2+P3) confirmed wildtype plants and tfiis mutants. The bottom panel shows PSTVd replication in wildtype and tfiis protoplasts. The sequence of P2 primer is derived from the inserted T-DNA, while the P1 and P3 primers are derived from the endogenous gene. WT, wildtype.
