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. 2020 Apr 6;8(4):524. doi: 10.3390/microorganisms8040524

Figure 2.

Figure 2

tRNACys gene region analysis for the Bacillus cereus group and some related species. 16S, 23S, 5S rRNA, and the cluster of 15 to 17 tRNA genes found downstream of the 5S rRNA gene are shown. tRNACys is in red color in the tRNA gene cluster and the orientation of three or four specific genes located downstream of tRNACys is shown in various other colors. For our PCR strategy, we used the second yebC/pmpR gene (which encodes a probable transcriptional regulatory protein) located downstream from tRNACys and between the first gene sequence (protein of unknown function) and the mutT-like gene (7,8-dihydro-8-oxoguanine-triphosphatase) indicated with purple and red colors, respectively. The sequences from these regions were obtained and analyzed in MEGA X, using the Neighbor-Joining method. Paenibacillus larvae was designated as the outgroup taxon.