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. 2020 Apr 6;8(4):524. doi: 10.3390/microorganisms8040524

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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B. cereus s.l. detection in commercial bee pollen. (A) Panel A show positive results of the tRNA^Cys-PCR analyses for isolates D8 to D14 and (B) panel B show negative results for tRNA^Cys-PCR (see text for bacterial species identified for lanes 1 to 6). Lane M contains a Thermo Scientific GeneRuler 1 Kb DNA ladder, lane C is the control (B. cereus ATCC 10876), and the arrows show the PCR amplification product. (C) Bacterial species identified by MALDI-TOF MS; numbers in parentheses indicate the total number of isolated colonies identified.