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. 2020 Apr 6;8(4):524. doi: 10.3390/microorganisms8040524

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic analysis in MEGA X, using the Neighbor-Joining method. tRNA^Cys- yebC/pmpR genes sequences of the isolates were compared with sequences of B. cereus group obtained by BLAST search in the NCBI, GenBank database (http://www.ncbi.nlm.nih.gov) and representative strains were selected for the analysis. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the taxa analyzed. Branches corresponding to partitions reproduced in less than 50% of the bootstrap replicates are collapsed. Group I contained almost exclusively species that were closely related to B. anthracis, while groups II, III, and IV contained species phylogenetically related to B. cereus and B. thuringiensis. The isolated colonies obtained are shown as D8 1517, D9 1517, D10 1517, D12 1517, D13 1517, and D14 1517 appearing within groups III and IV.