Skip to main content
. 2020 Apr 7;12(4):409. doi: 10.3390/v12040409

Figure 3.

Figure 3

Amino acid residue 120 in duck RIG-I CARD domains (122 in human RIG-I-CARD) contributes to species-specific MAVS-CARD–RIG-I-CARD interactions. (A) d2CARD A120D mutation decreases IFN-β promoter activity in DF-1 cells, while h2CARD wild-type and the complementary D122A mutant remain inactive. (B) RIG-I d2CARD A120D mutant is more active than wild-type d2CARD in HEK293T cells, while the corresponding mutation in h2CARD nearly abrogates IFN-β promoter activity. Graph bars show mean ± SD of three independent experiments (n = 3). Means were compared using one-way ANOVA with a Tukey’s multiple comparisons post-hoc test (****, p ≤ 0.0001). Total transfected DNA quantity was always kept constant with empty vector. (C,D) All GST-2CARD proteins are expressed in DF-1 cells (C) and HEK293T cells (D). Whole cell lysates were immunoblotted with anti-GST and anti-actin antibodies.